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in vitro transcribed translated (tnt) products  (Promega)

 
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    Structured Review

    Promega in vitro transcribed translated (tnt) products
    In Vitro Transcribed Translated (Tnt) Products, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/in+vitro+transcribed+translated+(tnt)+products/in+vitro+transcribed++translated/10__1074_slash_jbc__m010778200-99-14-16
    Average 90 stars, based on 1 article reviews
    in vitro transcribed translated (tnt) products - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: NBS1 is involved in DNA repair and plays a synergistic role with ATM in mediating meiotic homologous recombination in plants
    Article Snippet: .. PCR products were transcribed and translated in vitro (TnT kit, Promega) in the presence of 0.4 lCi ll)1 [35S]methionine (>1000 Ci mmol)1, MP Biomedicals Europe; http://www.mpbio.com). .. Then, 10 ll TnT mix was incubated with 10 ll of AtMRE11-bound or control paramagnetic beads in a final volume of 100 ll RS buffer for 10 min. Beads were washed twice with 1 ml RS buffer, once with 1 ml RS buffer + 500 mM NaCl, and once again with 1 ml RS buffer prior to elution in 50 ll tricine gel loading buffer (Bio-Rad) at 90 C for 10 min.

    Article Title: Identification of BRCA1 missense substitutions that confer partial functional activity: potential moderate risk variants?
    Article Snippet: .. The PCR products were transcribed and translated in vitro using the Promega TNT Coupled Reticulocyte Lysate System and sulfur-35 L-methionine (PerkinElmer, Melbourne, Australia) according to the manufacturer's instructions. ..

    In Vitro:

    Article Title: NBS1 is involved in DNA repair and plays a synergistic role with ATM in mediating meiotic homologous recombination in plants
    Article Snippet: .. PCR products were transcribed and translated in vitro (TnT kit, Promega) in the presence of 0.4 lCi ll)1 [35S]methionine (>1000 Ci mmol)1, MP Biomedicals Europe; http://www.mpbio.com). .. Then, 10 ll TnT mix was incubated with 10 ll of AtMRE11-bound or control paramagnetic beads in a final volume of 100 ll RS buffer for 10 min. Beads were washed twice with 1 ml RS buffer, once with 1 ml RS buffer + 500 mM NaCl, and once again with 1 ml RS buffer prior to elution in 50 ll tricine gel loading buffer (Bio-Rad) at 90 C for 10 min.

    Article Title: Identification of BRCA1 missense substitutions that confer partial functional activity: potential moderate risk variants?
    Article Snippet: .. The PCR products were transcribed and translated in vitro using the Promega TNT Coupled Reticulocyte Lysate System and sulfur-35 L-methionine (PerkinElmer, Melbourne, Australia) according to the manufacturer's instructions. ..

    other:

    Article Title: Second zinc finger mutants of thyroid hormone receptor selectively preserve DNA binding and heterodimerization but eliminate transcriptional activation.
    Article Snippet: Transcriptional regulation by thyroid hormone is mediated through its nuclear receptors (TRs), which bind to target responsive elements as homodimers or as heterodimers with 9-cis retinoic acid receptors (RXRs).. We examined the dimerization and functional properties of TRs containing mutations in the first and second zinc finger regions of the DNA binding domain.. Interestingly, a mutation (R158G) in the loop of second zinc finger, or a chimeric mutant in which the second zinc finger of the glucocorticoid receptor (GR) was substituted for that of the TR, did not form homodimer, but still bound as a heterodimer with RXRa.

    Article Title: Silver-haired bat rabies virus variant does not induce apoptosis in the brain of experimentally infected mice.
    Article Snippet: To examine whether induction of apoptosis plays a role in the pathogenesis of street rabies, we compared the distribution of viral antigens, histopathology, and the induction of apoptosis in the brain of mice infected with a street rabies virus (silver-haired bat rabies virus, SHBRV) and with a mouse-adapted laboratory rabies virus strain (challenge virus standard, CVS-24).. In ammation was identi ed in the meninges, but not in the parenchyma of the brain of mice infected with either CVS-24 or SHBRV.. Necrosis was present in numerous cortical, hippocampal, and Purkinje neurons in CVS-24-infected mice, but only minimal necrosis was identi ed in mice infected with SHBRV.



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    90
    Promega in vitro transcribed translated (tnt) products
    In Vitro Transcribed Translated (Tnt) Products, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/in+vitro+transcribed+translated+(tnt)+products/in+vitro+transcribed++translated/10__1074_slash_jbc__m010778200-99-14-16
    Average 90 stars, based on 1 article reviews
    in vitro transcribed translated (tnt) products - by Bioz Stars, 2026-09
    90/100 stars
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